Kanazawa University research: High sensitivity high throughput biosensor measures metabolite levels that indicate disease

Kanazawa University Japan United States of America United Kingdom

Fig. 1 Caption: Chemical structure of Pillar[n]arene and 1-methylnicotinamide (1-MNA).
Copyright 2024 Masaya Ueno

Fig. 2 https://nanolsi.kanazawa-u.ac.jp/wp/wp-content/uploads/eye-catch_Ueno.png
Fig. 2 Caption: Pillar[6]arene with 12 sulfonate groups (P6AS) specifically forms complexes with 1-methylnicotinamide (1-MNA)-a niacin metabolite-and works as a biosensor even in unpurified biological samples.
Copyright 2024 Masaya Ueno 

Glossary

Pillar[n]arenes

The pillar[n]arenes take their names from their pillar shapes and macrocyclic structures. The researchers were prompted to investigate the potential of P[6]AS as a 1-MNA detector following previous work by another group demonstrating the high binding affinity of P[n]AS for ammonium ions.

Binding affinity

The binding affinity refers to the strength of bond between the ligands on the protein and the candidate molecule, typically through intermolecular interactions such as ionic or hydrogen bonding or van der waals forces. The is a higher binding affinity when the attractive forces are higher.

Mass spectrometry

Mass spectrometers identify molecules based on their mass. The molecule is ionized and then accelerated through a field. The trajectory the molecule takes gives an indication of the charge mass ratio of the ion.

Reference

Masaya Ueno, Hiroki Sugiyama, Feng Li, Tatsuya Nishimura, Hiroshi Arakawa, Xi Chen, Xiaoxiao Cheng, Shinji Takeuchi, Yumie Takeshita, Toshinari Takamura, Sakae Miyagi, Tadashi Toyama, Tomoyoshi Soga, Yusuke Masuo, Yukio Kato, Hiroyuki Nakamura, Hiromasa Tsujiguchi, Akinori Hara, Atsushi Tajima, Moeko Noguchi-Shinohara, Kenjiro Ono, Kenta Kurayoshi, Masahiko Kobayashi, Yuko Tadokoro, Atsuko Kasahara, Mahmoud I. Shoulkamy, Katsuhiro Maeda, Tomoki Ogoshi, Atsushi Hirao A supramolecular biosensor for rapid and high-throughput quantification of a disease-associated niacin metabolite Analytical Chemistry 2024.

DOI?10.1021/acs.analchem.4c02653
URL: https://pubs.acs.org/doi/10.1021/acs.analchem.4c02653

Funding acknowledgments 

M.U. was supported by a Grant-in-Aid for Scientific Research (C) (23K06672) from the Ministry of Education, Culture, Sports, Science, and Technology, (MEXT), Japan. A.H. was supported by a Grant-in-Aid for Scientific Research (A) (19H01033) from MEXT and a Project for Cancer Research and Therapeutic Evolution (P-CREATE) (19cm0106104h0004) from the Japan Agency for Medical Research and Development (AMED). T.O. was supported by the CREST program of the Japan Science and Technology (JST) agency (JPMJCR18R3) and a Grant-in-Aid for Scientific Research (A) (22H00334) from MEXT. This work was supported by a WPI-NanoLSI Transdisciplinary Research Grant from Kanazawa University. NanoLSI is supported by the World Premier International Research Center Initiative (WPI), MEXT, Japan. This work was supported by WISE Program for Nano-Precision Medicine, Science, and Technology of Kanazawa University by MEXT. This work was also supported by MEXT Promotion of Development of a Joint Usage/Research System Project: Coalition of Universities for Research Excellence (CURE) Program (JPMXP1323015484).

Contact:

Kimie Nishimura (Ms)
Project Planning and Outreach
NanoLSI Administration Office, Nano Life Science Institute (WPI-NanoLSI)
Kanazawa University
Kakuma-machi, Kanazawa 920-1192, Japan
Email: [e-mail]
Tel: [phone]

About Nano Life Science Institute (WPI-NanoLSI), Kanazawa University

Understanding nanoscale mechanisms of life phenomena by exploring "uncharted nano-realms".

Cells are the basic units of almost all life forms. We are developing nanoprobe technologies that allow direct imaging, analysis, and manipulation of the behavior and dynamics of important macromolecules in living organisms, such as proteins and nucleic acids, at the surface and interior of cells. We aim at acquiring a fundamental understanding of the various life phenomena at the nanoscale.

https://nanolsi.kanazawa-u.ac.jp/en/

About the World Premier International Research Center Initiative (WPI)

The WPI program was launched in 2007 by Japan's Ministry of Education, Culture, Sports, Science and Technology (MEXT) to foster globally visible research centers boasting the highest standards and outstanding research environments. Numbering more than a dozen and operating at institutions throughout the country, these centers are given a high degree of autonomy, allowing them to engage in innovative modes of management and research. The program is administered by the Japan Society for the Promotion of Science (JSPS).

See the latest research news from the centers at the WPI News Portal: https://www.eurekalert.org/newsportal/WPI

Main WPI program site: www.jsps.go.jp/english/e-toplevel

About Kanazawa University

As the leading comprehensive university on the Sea of Japan coast, Kanazawa University has contributed greatly to higher education and academic research in Japan since it was founded in 1949. The University has three colleges and 17 schools offering courses in subjects that include medicine, computer engineering, and humanities.

The University is located on the coast of the Sea of Japan in Kanazawa – a city rich in history and culture. The city of Kanazawa has a highly respected intellectual profile since the time of the fiefdom (1598-1867). Kanazawa University is divided into two main campuses: Kakuma and Takaramachi for its approximately 10,200 students including 600 from overseas.

http://www.kanazawa-u.ac.jp/en/

 

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